Spirituality, in a narrow sense, concerns itself with matters of the spirit, a concept closely tied to religious belief and faith, a transcendent reality, or one or more deities. Spiritual matters are thus those matters regarding humankind's ultimate nature and purpose, not only as material biological organisms, but as beings with a unique relationship to that which is perceived to be beyond both time and the material world. Spirituality also implies the mind-body dichotomy, which indicates a separation between the body and soul.

As such, the spiritual is traditionally contrasted with the material, the temporal and the worldly. A perceived sense of connection forms a central defining characteristic of spirituality — connection to a metaphysical reality greater than oneself, which may include an emotional experience of religious awe and reverence, or such states as satori or nirvana. Equally importantly, spirituality relates to matters of sanity and of psychological health. Spirituality is the personal, subjective dimension of religion, particularly that which pertains to liberation or salvation (see also mysticism and esoterism) .

Spirituality as a way of life concerns itself with aligning the human will and mind with that dimension of life and the universe that is harmonious and ordered. As such spiritual disciplines (which are often part of an established religious tradition) enjoin practitioners (trainees or disciples) to cultivate those higher potentialities of the human being that are more noble and refined (wisdom and virtue). Accordingly, many spiritual traditions across diverse cultures share similar vocabulary. Terms such as the "path", the "work", the "practice" are universally applied to the ongoing discipline involved in transforming the coarser energies present in the human soul into more subtle and pleasing ones. As a spiritual practitioner one seeks to become free of the lesser egoic self (or ego) in favor of being more fully one's "true" "Self".

The spiritual and the religious

An important distinction exists between spirituality in religion and spirituality as opposed to religion.

In recent years, spirituality in religion often carries connotations of a believer having a faith more personal, less dogmatic, more open to new ideas and myriad influences, and more pluralistic than the doctrinal/dogmatic faiths of mature religions. It also can connote the nature of believers' personal relationship or "connection" with their god(s) or belief-system(s), as opposed to the general relationship with a Deity as shared by all members of a given faith.

Those who speak of spirituality as opposed to religion generally meta-religiously believe in the existence of many "spiritual paths" and deny any objective truth about the best path to follow. Rather, adherents of this definition of the term emphasize the importance of finding one's own path to whatever-god-there-is, rather than following what others say works. In summary: the path which makes the most coherent sense becomes the correct one (for oneself).