Electroporation is a dynamic phenomenon that depends on the local transmembrane voltage at each cell membrane point. It is generally accepted that for a given pulse duration and shape, a specific transmembrane voltage threshold exists for the manifestation of the electroporation phenomenon (from 0.5 V to 1 V). This leads to the definition of an electric field magnitude threshold for electroporation (E_th). That is, only the cells within areas where E≧E_th are electroporated. If a second threshold (E_ir) is reached or surpassed, electroporation will compromise the viability of the cells, i.e., irreversible electroporation.

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